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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two more splice variants had been claimed but specific nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines even more attainable optimization of cellular and immunoassay parameters to make certain the very best outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Facts Cyclin E belongs into the extremely conserved cyclin loved ones, whose users are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which lead for the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and capabilities being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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